|
KMID : 0361620070420010106
|
|
Journal of the Korean Orthopaedic Association
2007 Volume.42 No. 1 p.106 ~ p.114
|
|
|
Agonist for the Control of Apotosis through the Study of Cytokine Expression after Spinal Cord Injury in Rats
|
|
Yang Jun-Young
Riew K. Daniel
|
|
|
Abstract
|
|
|
Purpose : To analyze the cytokines that appear after a spinal cord injury in rats and to determine the agonists that regulate apoptosis.
Materials and Methods: Sixty female Sprague-Dawley rats were anesthetized, and a laminectomy was performed at the 9th thoracic vertebra. The spinal cord injury was induced by dropping a 10 gm weight at a height of 20 mm. The subjects were divided into 5 groups. Group I was administered aminoguanidine, group II was administered GM-CSF, group III was administered riluzole, group IV was administered erythropoietin, and group V was administered methylprednisolone. A control group was administered normal saline. The results were assessed using the Tarlov motor grading method. In 1, 3, 5 and 7 days after the spinal cord injury, the rats were sacrificed and evaluated using the syringomyelic cavity size. Immunohistochemical staining for e-NOS and RT-PCR for XIAP were also performed.
Results: Groups I, III, and V showed significantly different results in terms of the motor recovery and inhibition of increase in the syringomyelic cavity compared with the other groups (p 0.05). The e-NOS activity was observed in groups I, III, and V. The other groups showed almost no e-NOS activity. On the RT-PCR, groups I, III, and V showed significantly different results in terms of XIAP expression with time compared with the other groups.
Conclusion: Steroids, NOS inhibitors and sodium channel inhibitors appear to be important factors
for regulating apoptosis in the early stage of a spinal cord injury. Further study will be needed to
develop new pharmaceuticals with synergic effects on spinal cord injuries.
|
|
|
KEYWORD
|
|
|
Apoptosis, Spinal cord injury
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
  
|
|